Composite

Part:BBa_M1535:Design

Designed by: Sarah Allred   Group: UtahState_BE5930_-_S11   (2012-04-20)


Thermal Hysteresis (Antifreeze) Protein (variant YL1) with 8-His tag with secretion tag and GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4579
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2246
    Illegal BglII site found at 2909
    Illegal BamHI site found at 5098
    Illegal XhoI site found at 4999
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1176
    Illegal AgeI site found at 496
    Illegal AgeI site found at 4415
    Illegal AgeI site found at 4472
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

See subparts


Source

See subparts

References